Enzymes are sensitive to environmental conditions such as temperature, pH, and substrate concentration.
These factors affect the enzyme's activity and efficiency.
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Temperature: Each enzyme has an optimal temperature at which it functions most efficiently; too
high or too low can denature the enzyme.
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pH: Enzymes also have an optimal pH range; deviations from this range can reduce activity or
denature the enzyme.
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Concentration: Increasing substrate concentration increases reaction rate to a point, after which
the enzymes become saturated.